General Cell Lines and Cell Cultures
Eurofins Discoverx PathHunter CHO-K1 CCR3 β-Arrestin Cell Line, 2 vials. (93-0629C2)
The PathHunter CCR3 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. CCL13/MCP-4) based activation of CCR3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CCR3 and EA-tagged β-Arrestin-2. Activation of CCR3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Biochain Institute Inc Paraffin Tissue Section Panel - Human Tumor Same Tumor Type, Different Donors (5 tissues), 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures.The tissues are immediately put in liquid nitrogen after being excised, and then identified by a board-licensed pathologist. Patient demographic and disease related history data are available. he tissue sections are approximately 5-10 um thick.
Eurofins Discoverx PathHunter CHO-K1 LXRβ Protein Interaction Cell Line, 2 vials. (93-0442C2)
The PathHunter LXRβ Protein Interaction cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting NHR activation and recruitment of cofactors. This cell line enables assessment of ligand (e.g. oxysterols) based NHR activation via detection of SRCP (steroid receptor co-activator peptide) recruitment. The included cell line overexpresses ProLink-tagged LXRβ NHR and EA-tagged steroid receptor co-activator peptide (SRCP). Upon activation of LXRβ, induced interaction with SRCP leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/nhr.
Eurofins Discoverx PathHunter U2OS mPD-L1 Ligand Cell Line, 2 vials. (93-1159C3)
The PathHunter mPD-L1 Ligand cell line is a stable, engineered cell line designed as a companion product for use with mPD-1 Checkpoint Signaling Cell Lines. When co-cultured, the mPD-L1 expressed in this cell line will induce activity of mPD-1 receptors, which may be assayed through SH2 domain protein recruitment. The cell line included overexpresses mPD-L1 cell surface ligand. When co-cultured with the mPD-1 Checkpoint Signaling Cell Line, the mPD-L1 ligand binds to the checkpoint receptor, inducing receptor activity. This receptor activity leads to recruitment of the relevant SH2 domain protein to the checkpoint receptor inside the receptor cell line and production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/checkpoint.
Eurofins Discoverx PathHunter CHO-K1 DRD4 β-Arrestin Cell Line, 2 vials. (93-0690C2)
The PathHunter DRD4 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. dopamine) based activation of DRD4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged DRD4 and EA-tagged β-Arrestin-2. Activation of DRD4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 PPARγ Protein Interaction Cell Line, 2 vials. (93-0390C2)
The PathHunter PPARγ Protein Interaction cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting NHR activation and recruitment of cofactors. This cell line enables assessment of ligand (e.g. prostaglandin J2, fatty acids) based NHR activation via detection of SRCP (steroid receptor co-activator peptide) recruitment. The included cell line overexpresses ProLink-tagged PPARγ NHR and EA-tagged steroid receptor co-activator peptide (SRCP). Upon activation of PPARγ, induced interaction with SRCP leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/nhr.
Eurofins Discoverx PathHunter CHRM4 Total Internalization U2OS Cell Line, 2 vials. (93-0902C3)
The PathHunter CHRM4 Total Internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of CHRM4 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged CHRM4 and a ProLink-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the CHRM4 receptor to the early endosome leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
ABclonal Technology ADORA2A Rabbit pAb
This gene encodes a member of the G protein-coupled receptor (GPCR) superfamily, specifically an A2A adenosine receptor. It responds to adenosine, activating G(s) and G(olf) proteins to increase intracellular cAMP levels. The receptor plays key roles in regulating cardiac rhythm, circulation, cerebral and renal blood flow, immune function, pain, and sleep. It is involved in conditions like inflammatory diseases and neurodegenerative disorders. Alternative splicing produces multiple transcript variants, including a read-through transcript with the upstream SPECC1L gene, thought to be non-coding.
Eurofins Discoverx Ready-to-Assay EP1 Prostanoid Receptor Frozen Cells, 2 Vials of Cells. (HTS099RTA)
The Ready-to-Assay EP1 Prostanoid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. prostaglandin E2) based activation of PTGER1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PTGER1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of PTGER1, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx Ready-to-Assay EP3 Prostanoid Receptor Frozen Cells, 2 Vials of Cells. (HTS092LRTA)
The Ready-to-Assay EP3 Prostanoid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. prostaglandin E2) based activation of PTGER3 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PTGER3 alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of PTGER3, the resulting calcium activity can be assayed in either detection mode. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
ATCC Quantitative Synthetic; Chlamydia trachomatis; LGV Type 1 DNA
Quantitative Synthetic Chlamydia trachomatis LGV Type 1 DNA can be used for assay development, verification, and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine bacterial load. Preparation includes fragments from MOMP, 16S rRNA, pmpH, dnaB, putative virulence plasmid integrase regions, and conserved hypothetical virulence plasmid protein.
Biotium CD8A (Cytotoxic- & Suppressor T-Cell Marker) (C8/1779R), CF647 conjugate, 0.1mg/mL
CD8 is a cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells. For mature T-cells, CD4 and CD8 are mutually exclusive, so anti-CD8, generally used in conjunction with anti-CD4. It is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral T-cell lymphomas are CD4 /CD8-. Anaplastic large cell lymphoma is usually CD4 and CD8-, and in T-lymphoblastic lymphoma/leukemia, CD4 and CD8 are often co-expressed. CD8 is also found in littoral cell angioma of the spleen.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note:
Biochain Institute Inc Frozen Tissue Section - Human Adult Normal: Stomach, 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's Frozen Tissue Slides are ideal for rapidly identifying cellular localization of RNA or protein. A single human adult normal tissue with 5-10 um thickness is mounted on a positively charged glass slide. The slides are fixed and dehydrated with acetone for consistent results with in situ hybridization and immunohistochemistry. More organs may be available upon request.
Biochain Institute Inc Matched Pair - Paraffin Tissue Section - Human Primary Tumor and Normal (PP): Kidney, 5 x2 slides /PK
BioChain's human paraffin tissue section (FFPE) matched pair products include: Primary Pair (PP), Primary and Metastatic Pair (PM). PP consists of paraffin tissue sections of primary tumor and its adjacent normal tissue; PM consists of paraffin tissue sections of primary tumor and corresponding metastatic tumor. Paraffin tissue sections in each pair are prepared from the same donor. This product line is ideal for localization of tumor- specific genes and tumor metastatic genes.
Biochain Institute Inc Matched Pair - Total Protein - Human Primary Tumor and Metastatic Tumor Tissue (PM): Breast, 0.2 x2 mg /PK
BioChain's human total protein matched pair products include: Primary Pair (PP), Primary and Metastatic Pair (PM). PP consists of total protein isolated from primary tumor and its adjacent normal tissue; PM consists of total protein from primary tumor andcorresponding metastastic tumor. Total proteins in each pair are prepared from the same donor. This product line is designedfor identifying tumor-specific genes and tumor metastasis genes.